Over the summer I have been working at Emory University with Dr. Tansey and other lab members looking for potential causes of Parkinson’s disease (PD). Parkinson’s disease is an illness that damages important parts of the brain and nervous system. This can cause a loss in efficient body movement. We believe that a specific protein, something that the body naturally makes, called LRRK2 may play a role in this disease because there is an increased amount found in PD patients compared to healthy controls. Therefore, we have studied this protein by trying to pin point its location in the body and learning how it causes other PD symptoms such as inflammation. This research will not only provide answers for PD, but can be used to learn more about other neurodegenerative diseases. The goal is to one day cure such debilitating illnesses for everyone who is unfortunate to develop them.
Realities of Research
One of the biggest things I have learned about working in a lab is that your plan of action can suddenly change, while the goal stays the same. However, there are many times where you must go back to the “drawing board” and erase or insert something new. To me, going back and finding out that you have to try something new is a good thing. You are improving on your research and hopefully it will bring you closer to significant results. I also had other learning opportunities such as improving on specific lab techniques. During my BCA assay (Bicinchoninic Acid assay), which is a protocol used to standardize amounts of proteins in a sample, I had to perfect my pipetting skills for the assay to work. I practiced for hours to get the exact amount of solution every time I withdrew from a tube before I actually ran the real assay. This summer, my project started off bumpy because of an experiment not going as planned. I was taking tissue sections of kidney to stain and investigate for the colocalization of LRRK2 with the primary regulator NFAT (Nuclear Factor of Activated T-cells) and pNFAT (NFAT promoter) status in the nucleus. We were looking at these specific cells because they are involved with the immune system which LRRK2 seems to help regulate. However, after my staining protocol, the kidney cells would essentially combust. After many days of trial and error, we decided that there was not enough knowledge of kidney staining in our lab, and instead of spending time and resources to figure it out we moved on and started looking at a new location. There were plenty of times where we had to re-evaluate our plan, and as I said before I only see them as learning opportunities. Sometimes research is not always going to be straight forward where you make a hypothesis, go through an experiment, and at the end collect an answer. It can take a lot of time and creative thinking to get where you need to go.
Life of a Scientist
Being a part of a lab is great for many reasons. One reason is that I prefer the type of work style that it offers. Those aspects include being able to have flexibility within your schedule and not having to be constantly micromanaged. Yet, I am still offered enough guidance to steer me on the right track. I love that every day is another day pushing towards a goal that you most likely had a hand in setting. I am also surrounded by people who all want to see each other succeed. Although everyone in the lab may be working on their own personal agendas, people are constantly helping others with their projects whether it’s by lending their skills for a certain assay or giving an extra hand to make timely experiments go by faster. I would go on to say that the entire science community within your field begins to feel more familiar as your work progresses, as well. I value this idea of a connected community within my workspace. Overall, my experiences in my lab this summer were positive and resourceful. Of course, I have witnessed complications occurring in the lab such as assays not going as planned or having to re-due them because of minor mistakes. Human error constantly occurs and I have learned that you can only work with it and make sure to try as hard as possible to get accurate, significant results. Knowing that I could make a career into doing what I love, conducting science and answering important questions that benefit humanity, gives me the motivation of becoming a neurologist in the future.