The spectacular process of human reproduction is complex, time consuming, and, above all, fascinating! Much has been learned over the years dealing with the mechanisms of pregnancy in many of Earth’s lifeforms. The research on genetics, like uncovering the entire human genome, makes incredible strides toward fully grasping why certain physiological processes happen. However, there are still numerous question marks, specifically speaking about women’s health during pregnancy and after, that require research and understanding. Full efforts are being undertaken that aim to ultimately lead to safer pregnancies, better means of treating diseases, and developing new techniques.
Preeclampsia is a disorder during pregnancy characterized by high blood pressure and excess protein excretion. Because the condition is not entirely understood, treatment options are far and few between for women suffering. Currently, the only remedy is a low-dose treatment of aspirin. The effects and mechanisms of this aspirin treatment is not completely understood either, so the purpose of my study is to attempt to demystify the workings of the treatment. Specifically, I am targeting human trophoblast cells, the major cell type involved in the development of the placenta, an organ that provides nutrition to the developing fetus. By varying different doses of aspirin, I am examining the changes, or lack of change, in the trophoblast DNA. If changes are observed, we will have knowledge on how and if aspirin will help women suffering from preeclampsia, which will ultimately lead to a safer pregnancy for both the mother and child.
Realities of Research
It has been an exciting experience working in a research lab. Not only have I learned valuable techniques, but I am directly impacting the future of medicine, even if it is in a seemingly small way. I have been surprised by the level of attention and precision that is addressed when conducting research. I always knew that attention to detail was important, but the extent to this precision that I have been performing has shocked me along with how these techniques were practiced. For example, RNA isolation is a delicate, yet simple process that requires attention and a good grasp on how to pipette well. If a step is skipped, such as forgetting to add the homogenate additive, then the RNA yield could be put at risk. It is too early in my research stages where results and conclusions can be made. Typically, one trial of cell growth requires one full week, so multiply one trial by the many that we are attempting and the overall experiment becomes lengthy.
Life of a Scientist
The day-to-day life of a young scientist has been exciting. While not all of the parts of my day are groundbreaking and entertaining, it is still a rewarding process. I usually begin my day with notebook entries, planning, and reading up on current events in my field. My research involves a fairly strict time schedule, so in the afternoon, the experimenting and cell ‘farming’ as I call it, can begin. I was surprised by the equipment that I have at my fingertips. Nothing is more thrilling than looking through a $45,000 microscope or running a 6 well plate through a machine that you can’t even pronounce. The best part so far for me has been the adjusting to a real life laboratory. I have begun to entertain the idea of having my own lab in the future, and becoming familiar with how a lab is run has been a wonderful experience. The worst part has been the waiting that is required between experiments. It makes me wish I had a magic wand that would make the cells grow and be ready for testing at the flick of my wrist. It has been so wonderful working with everyone in my lab. I love the feeling of having an independent project, but still being under a larger umbrella of research with my coworkers where we can discuss information and findings.