My research project explores the possible future uses of the alternative medicine Astragalus, which is a Chinese herb known for its immune stimulating effects, such as decreasing melanoma tumor growth. I am specifically looking for immune system responses in transparent roundworms called C. elegans. I am treating the worms with different concentrations of the Astragalus medicine, then observing how their immune system pathways have reacted. My research expands the information available on alternative medicines and their validity. This project could lead to further, more specific studies as well as studies on other animals and potentially humans. Astragalus could be proven to be a viable medication for disease or replace current cancer treatments and medications.
Working in a research lab has proved to be very interesting and exciting. I started out the summer unsure of myself and the techniques involved with my project. There were many standard procedures and instruments I needed to familiarize myself with to successfully and effectively perform tests. Over time I became more comfortable and could do most tests and procedures on my own, only after being instructed by my research host, of course. My research was unsuccessful at first as I couldn’t get the desired result from polymerase chain reaction (PCR) with the house-keeping gene actin. We reran the end-point PCR and ran another gel but still had no results. After taking another step back and rerunning the cDNA synthesis with my RNA sample, we finally had a successful PCR result. I know there are many techniques I have yet to master in my research journey.
I was surprised by how long certain experiments took and how much everyday maintenance was required to continue working with the worms. Much of my time was spent counting the worms for survival assays or synchronizing the worms to prepare for a test. Also, there was constant upkeep of Nematode Growth Media plates and E. coli stock. I also had to adjust to working independently on my research as opposed to working in a class. I had to prepare all the materials I needed and go through the steps on my own instead of having everything laid out for me. The best part of research was getting successful results and making progress, the worst part was becoming frustrated when results were undesirable or no results were produced at all.